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Fluorescence In Situ Hybridization (FISH)

BioVantra offers a continually expanding selection of highly specialized diagnostic, prognostic, and predictive testing technologies and services to urologists whose patient populations require more advanced information for treating cancer and other complex diseases. In addition, with the advent of the human genome project, more sophisticated technologies are becoming a reality, offering increasing possibilities in personalized healthcare.

Automated Fluorescent Scanning & Analysis of Target Cells in Suspension, Previously Stained with a Different Marker

Target FISH

BioVantra’s Target FISH (T-FISH) application provides a genetic content analysis (FISH) of a specific, pre-selected subset of target cells. First, the application scans slide stained with Papanicolaou stain, Immunofluorescent antibodies (IFL), or FISH probes to detect and select specific cell types/groups. Then, it rescans the same slides after de-staining and is stained again with different Immunofluorescent antibodies or FISH probes. Finally, the T-FISH application matches the two scans to present two images of the same cell for each cell in the sample (morphology/FISH or IFL/FISH or FISH/FISH).

Examples of Papanicolaou-stained urine cytology and corresponding UroVysion FISH (Chromosomes 3 in red, 7 in green, 17 in aqua, and 9p21 in gold)

a. Benign “umbrella” cell with pronounced reactive changes in a patient with irritative voiding symptoms. Highly increased number of all FISH signals due to repeated doubling of the genome (i.e., endoreplication), but no unbalanced copy number changes and no 9p21 deletion.

b. Sheets of mildly atypical urothelial cells in a voided urine sample. Normal FISH result (two copies per chromosome), consistent with reactive changes.

c. Atypical urothelial cells suspicious for high-grade urothelial carcinoma. Positive UroVysion FISH result with increased copy numbers of all three chromosomes and homozygous deletion of 9p21.

d. UroVysion FISH positive atypical urothelial cells with increased copy number of all five chromosomes but no 9p21 deletion.

Target FISH